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. 2018 Nov 9;8(20):5575–5592. doi: 10.7150/thno.27372

Figure 6.

Figure 6

Phosphorylation of GSK3β by LiCl manifests a stronger effect than TNSALP mimic on rescuing the impaired function of HPP BMMSCs. (A) Alizarin red staining and quantification of mineralized nodules performed at day 28 after osteogenic induction (OS) in Wnt3a-treated (25 ng/mL) and LiCl-treated (10 mM) HPP BMMSCs. Expression levels of Runx2 and OCN were examined by western blotting. (B) Oil Red O staining and quantification of fat depots were performed at day 14 after adipogenic induction. PPAR-γ expression was examined by western blotting. Scale bars, 100 μm. (C) Alizarin red staining and quantification of mineralized nodules performed at day 28 after OS in TNSALP-treated (10 U/mL) and LiCl-treated (10 mM) HPP BMMSCs. Expression levels of Runx2 and OCN were examined through western blotting at day 7 after induction. (D) Oil Red O staining and quantification of fat depots were performed at day 14 after adipogenic induction (AD). PPAR-γ expression was examined at day 7 after induction by western blotting. Scale bars, 100 μm. HPP n = 2 per group. Data shown as mean ± s.d. for triplicate samples from a representative experiment. *P < 0.05. One-way analysis of variance (ANOVA).