Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Laboratory-Scale Purification of AAV9-dsLuc or AAV9-ssLuc
(A–F) Purity assessment of (A–C) AAV9-dsLuc and (D–F) AAV9-ssLuc. The preparation of final rAAV9 product was analyzed by (A and D) 5%–20% (v/v) gradient gel SDS-PAGE with Oriole fluorescent staining, (B and E) western blotting, and (C and F) EM (negative staining). Lane 1 and lane 2, final purified rAAV9 product. The three bands represent the AAV9 capsid proteins VP1 (82 kDa), VP2 (67 kDa), and VP3 (60 kDa).
