Table 2.
Levels of Contaminating Human Genomic DNA Quantified in the Final Preparation of AAV9-dsEGFP
| rAAV9 (1.0 × 109 v.g.) |
Human Genomic DNA Contamination |
|
|---|---|---|
| Benzonase Treatment | Final Producta | Post-TFF |
| +b | <1 copyc | <1 copyc |
| −d | <1 copyc | 2.6 copies |
a
Final products of three trials (AAV9-dsEGFP) were examined.
b
Benzonase treatment (+) represents the contaminating human genomic DNA in rAAV9 particles.
c
Less than one copy means below the detection limit.
d
Benzonase treatment (−) represents the contaminating human genomic DNA in and outside of rAAV9 particles.