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. Author manuscript; available in PMC: 2018 Dec 3.
Published in final edited form as: Nat Med. 2017 Apr 10;23(6):763–767. doi: 10.1038/nm.4322

Extended Data Figure 6. Efficacy of immunization with 10 IFU 10-del virus.

Extended Data Figure 6.

(a) Viremia after immunization with 10 IFU of WT or 10-del ZIKV. Three-week-old A129 mice (n=5) were immunized with 10 IFU WT or 10-del virus via the S.C. route. Viremia were quantified by immunostaining focus assay from day 4 to 7. L.O.D., limit of detection. (b) Pre-challenge neutralization antibody titers. Three-week-old A129 mice (n=5) were immunized with 10 IFU 10-del ZIKV and PBS via the S.C. route. On day 28 post-immunization, mouse sera were quantified for ZIKV neutralizing antibody titers. (c) Viremia after challenge with epidemic ZIKV (Puerto Rico strain PRVABC59). On day 28 post-immunization, the mice were challenged with 1×106 IFU of ZIKV via the I.P. route. On day 2 post-challenge, viremias were quantified using an immunostaining focus assay.