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. Author manuscript; available in PMC: 2018 Dec 3.
Published in final edited form as: Nat Med. 2017 Apr 10;23(6):763–767. doi: 10.1038/nm.4322

Figure 2. Characterization of 3’UTR mutants in the A129 mouse model.

Figure 2.

(a) Experimental scheme. In two separate experiments, three-week old A129 mice (n=8) were immunized via the S.C. route with 1×104 IFU WT and mutant viruses. The immunized mice were monitored for weight loss (b), survival (c), and viremia (d). Weight loss is indicated by percentage using the weight on the day before immunization as 100%. Viremias were quantified by an immunostaining focus assay from day 2 to 4 post-infection. (e) Pre-challenge neutralization antibody titers. On day 28 post-immunization, mouse sera were measured for neutralizing titers using a mCherry ZIKV infection assay (Extended data Fig. 4). (f) Post-challenge viremia. On day 28 post-immunization, mice were challenged with 1×105 PFU parental virus (ZIKV strain FSS13025) via the I.P. route. Viremia on day 2 post-challenge was quantified using the immunostaining focus assay. (g) Post-challenge neutralization antibody titer. On day 28 post-challenge, mouse sera were quantified for neutralizing titers using the mCherry ZIKV infection assay. L.O.D.: limit of detection.