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. Author manuscript; available in PMC: 2018 Dec 3.
Published in final edited form as: Nat Med. 2017 Apr 10;23(6):763–767. doi: 10.1038/nm.4322

Extended Data Figure 4. Construction of mCherry ZIKV.

Extended Data Figure 4.

(a) Schematic genome of a mCherry ZIKV. A DNA fragment (encoding the first 25 amino acids of C gene, the mCherry gene, and the foot-and-mouth virus 2A protein) was in-frame fused with the open-reading-frame of ZIKV genome. (b) The mCherry expression in Vero cells transfected with mCherry ZIKV RNA. The expression of mCherry in transfected Vero cells was analyzed by a fluorescent microscopy at the indicated days post-transfection. The mCherry ZIKV was used to estimate antibody neutralization titers of mouse sera, as described in Methods.