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Methane-rich saline attenuated kidney histopathological damage in CLP mice. (a) H&E staining was performed on kidney tissue slices 24 h after CLP. (b) The infiltrated neutrophil granulocytes were counted. (c) PAS staining was performed on kidney tissue slices 24 h after CLP. (d) The tubular damage scores of kidney tissue were calculated. Representative sections; original magnification, 200x. ∗P < 0.05, ∗∗P < 0.01, and ∗∗∗P < 0.001.
