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. 2018 Nov 19;2018:7897263. doi: 10.1155/2018/7897263

Figure 2.

Figure 2

Tofacitinib regulates IFN-γ- and IL-22- but not TNF-α-induced signaling molecules in psoriatic keratinocytes. Protein extracts obtained from psoriatic keratinocytes pretreated with 5 μM tofacitinib or vehicle alone and then stimulated or not with IFN-γ, IL-22, or TNF-α for 20 min were used on a PathScan intracellular signaling array, which allows the simultaneous detection of 18 signaling molecules when phosphorylated or cleaved. They include ERK1/2, STAT1, STAT3, Akt (Thr308 and Ser473 phosphorylation), AMPKa, mTOR, HSP27, Bad, p53, p38, SAPK/JNK, PARP, and caspase 3. Developed slides were acquired at ChemiDoc system. Graphs represent densitometric analyses of the indicated proteins. Data are expressed as mean ± SD fold induction (F.I.) calculated relatively to the untreated samples, which were given a value of 1. Protein panel was analysed in two assays with two different keratinocyte strains. Each value was normalized to an internal positive control. ∗p < 0.05 for samples treated with tofacitinib vs. untreated, in the presence of cytokines.