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The level of spleen lymphocyte culture supernatant IFN-γ (a), IL-2 (b), IL-4 (c), and IL-10 (d) in immunized mice as measured by ELISA. Spleen lymphocytes were obtained on days 0, 14, 28, 42, and 56 after the first immunization. Inset shows the concentration of four groups at different time points. ∗Represents the difference between groups. p < 0.05. NS = nonsignificant.
