Table 3.
Primers used for real-time quantitative PCR
| Gene | Primer sequence, 5→3′ | Product size, base pair | Accession no. | |
|---|---|---|---|---|
| Forward | Reverse | |||
| IL-1β | TTGAATTCGAGTCTGCCCTGT | CCCAGGAAGACGGGCTTT | 76 | NM_214055 |
| IL-6 | GGCTGTGCAGATTAGTACC | CTGTGACTGCAGCTTATCC | 124 | AF518322 |
| IL-10 | TGGGTTGCCAAGCCTTGT | GCCTTCGGCATTACGTCTTC | 61 | L20001 |
| IL-17 | CCAGACGGCCCTCAGATTAC | CACTTGGCCTCCCAGATCAC | 103 | AB102693 |
| IL-22 | AAGCAGGTCCTGAACTTCAC | CACCCTTAATACGGCATTGG | 133 | AY937228 |
| Occludin | TCGTCCAACGGGAAAGTGAA | ATCAGTGGAAGTTCCTGAACCA | 95 | NM_001163647 |
| Claudin-3 | TTGCATCCGAGACCAGTCC | AGCTGGGGAGGGTGACA | 85 | NM_001160075 |
| ZO-11 | CTCTTGGCTTGCTATTCG | AGTCTTCCCTGCTCTTGC | 197 | XM_003353439 |
| RPL192 | AACTCCCGTCAGCAGATCC | AGTACCCTTCCGCTTACCG | 147 | AF435591 |
1ZO-1 = zonula occluden-1.
2RPL19 = ribosomal protein - L19.