Table 2.
Gene primer sequences used for quantitative RT-PCR in laying ducks
| Gene1 | Primer sequence (5′–3′) | Product size (bp) | Accession number |
|---|---|---|---|
| COX-1 | Forward: GCAGTGAACCCGTGTTGCTATTAC | 252 | XM_021278679.1 |
| Reverse: GAGCCTCATCAGCGTGTCCCT | |||
| COX-2 | Forward: AAACAGGAGCATCCAGAGTG | 216 | XM_005015351.3 |
| Reverse: GTGCCAGTGGTACAGGGTAT | |||
| NOX-1 | Forward: TCGCCTCCATCCTGAAGTCC | 256 | XM_021270354.1 |
| Reverse: GTGTCCGTGGCAGTGTCGTG | |||
| NOX-4 | Forward: AGCCACCCATTCACCCTTAC | 249 | XM_005029579.3 |
| Reverse: AATACCACCAGCCACGCAGA | |||
| 5-LOX | Forward: AATACCAAAGCCCGAGAACA | 133 | XM_005025227.3 |
| Reverse: GGAAGCAGAGTGAGCTGTAAGTC | |||
| CYP7A1 | Forward: ATCAGACTTTCATTAGAACCCTTCA | 169 | NM_001310351.1 |
| Reverse: GACTCAAACATCACCTGGCAAC | |||
| GR | Forward: CCCATCTGGACTACAGCAACA | 182 | XM_005025125.3 |
| Reverse: CTTCATCACGCACTTCACCTTC | |||
| MnSOD | Forward: CACCACAGCAAGCACCACG | 174 | XM_005015908.2 |
| Reverse: AGGCGAAAGATTTGTCCAGAA | |||
| CAT | Forward: AGATATGGTATGGGACTTTTGGAG | 180 | XM_013091878.2 |
| Reverse: TCAGTCTTCACATGAAACTTGCA | |||
| Complex Ι | Forward: GTCACCACCCTGGATAACGG | 250 | XM_005023916.3 |
| Reverse: GTCACCACCCTGGATAACGG | |||
| CoQ10 | Forward: TGTTTCACTGTTAGGCATTGTATTG | 146 | XM_021274031.1 |
| Reverse: GACAACTCATTCCCTCCTTTCA | |||
| β-actin | Forward: GCTATGTCGCCCTGGATTT | 174 | EF_667345.1 |
| Reverse: GGATGCCACAGGACTCCATAC |
1 COX-1, cyclooxygenase-1; COX-2, cyclooxygenase-2; NOX-1, NADPH oxidase-1; NOX-4, NADPH oxidase-4; 5-LOX, 5-lipoxygenase; CYP7A1, cytochrome P450 7A1; GR, glutathione reductase; Complex I, NADH dehydrogenase subunit Ι; CoQ10, Coenzyme Q10.