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. 2018 Nov 28;13:7939–7957. doi: 10.2147/IJN.S182611

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© 2018 Mosselhy et al. This work is published and licensed by Dove Medical Press Limited

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Malformations of zebrafish embryos/larvae.

Notes: Microscopic images (3X objective) of different developmental stages of zebrafish embryos/larvae 24 hpf (A), 48 hpf (B), 72 hpf (C), and 96 hpf (D). The control group presents the normal development of the embryos/larvae with no malformations (score: 0). The embryos treated with the SiO₂-G nanohybrids (500 and 1,000 µg/mL) show a range of malformations, such as YSE or YND, SD, SG, TM, and PE. The severity of malformations is scored (1–4). The highest score, 4, is marked for the DE.

Abbreviations: DE, degenerated embryos; PE, pericardial edema; SD, spinal deformity; SG, stunted growth; SiO₂-G, silica–gentamicin; TM, tail malformation; YND, yolk not depleted; YSE, yolk sac edema.