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. 2018 Nov 28;13:8087–8094. doi: 10.2147/IJN.S188074

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© 2018 Cuellar et al. This work is published and licensed by Dove Medical Press Limited

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(A) Antimicrobial activity against Escherichia coli of magnetite (brown) and BUF2-magnetite (500 μg/mL, green; 100 μg/mL, purple) as prepared by conjugation via glutaraldehyde. BUF2-magnetite as prepared by conjugation via EDC at the same concentrations (blue and red, respectively). Comparison with the antimicrobial activity of BUF2 at 100 μM (orange) and 3.125 μM (black). The positive control was Escherichia coli in gentamicin at 10 μM, and suspension containing only cells was the negative control. (B) Actual image of the microplate where the antimicrobial assay was conducted. BUF2 at 100 μM and the positive control showed insignificant turbidity when compared with the rest of the treatments.

Abbreviations: BUF2, buforin II; EDC, N-[3-(dimethylamino)-propyl]-N′-ethylcarbodiimide hydrochloride; Mag, magnetite; MBE, BUF2-magnetite conjugated with EDC; MBG, BUF2-magnetite conjugated with glutaraldehyde.