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. 2018 Mar 14;103(5):1842–1849. doi: 10.1210/jc.2017-01585

Figure 3.

Figure 3.

The PR agonist R5020 induces RANKL but not RANK gene expression. (A and B) Total RNA was extracted from leiomyoma explants treated with R5020 for 24 hours (A) or 48 hours (B). RANKL mRNA was quantified by using qRT-PCR; n = 3 independent tissue samples. (C) RANK mRNA was quantified in explants treated with R5020 for 48 hours; n = 3. (D) R5020 significantly induced RANKL expression in LICs. LSCs, LICs, and LDCs were sorted from leiomyoma explants treated with R5020 for 24 hours, and then RANKL mRNA level was quantified in each cell population. Data show a representative experiment performed in triplicate from three independent tissue samples. The error bars are the standard error of the mean. NS, not significant.