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Treatment of the total leiomyoma cells with RANKL increased cyclin D1 expression that was suppressed with the RANKL/RANK pathway inhibitor RANK-Fc. Upper panel shows immunoblot densities quantified with ImageJ software. Lower panel shows images of representative immunoblots. Experiments were repeated with cells from five patient samples. The error bars are the standard error of the mean. RFc, RANK-Fc; RL, RANKL.
