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. Author manuscript; available in PMC: 2018 Dec 3.
Published in final edited form as: J Steroid Biochem Mol Biol. 2010 Apr 9;121(3-5):538–545. doi: 10.1016/j.jsbmb.2010.03.068

Table 1.

Comparison of urinary estrogen/estrogen metabolite (EM) measurement by RIA/ELISA and LC/MS2: Spearman correlations and absolute concentrationsa.

EM Premenopausal luteal phase women N = 264 Premenopausal non-luteal phase women N=98 Postmenopausal women N =168
Spearman correlations
RIA and LC/MS2
 Estrone 0.94 0.96 0.79
 Estradiol 0.91 0.95 0.63
 Estriol 0.94 0.94 0.73
ELISA and LC/MS2
 2-Hydroxyestrone 0.81 0.89 0.37
 16α-Hydroxyestrone 0.86 0.89 0.62
Geometric mean concentrations pmol/mg creatinine
RIA/ELISA LC/MS2 RIA/ELISA LC/MS2 RIA/ELISA LC/MS2
RIA and LC/MS2
 Estrone 41.9 23.4 27.9 14.6 6.9 2.6
 Estradiol 17.6 10.9 12.0 7.7 2.1 1.5
 Estriol 77.2 55.5 50.1 31.2 12.9 5.7
ELISA and LC/MS2
 2-Hydroxyestrone 47.8 24.6 31.0 13.8 18.6 2.9
 16α-Hydroxyestrone 32.2 11.0 23.8 6.5 14.1 1.2
a

Subjects are Asian–American women, aged 20–55 years, selected as controls for a population-based case-control study of breast cancer [29]. 12 h overnight urines were collected.