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. 2018 Nov 26;9:2718. doi: 10.3389/fimmu.2018.02718

Figure 3.

Figure 3

Cellular source of IL-6 in HDM-induced asthma. (A) Frequency (%) of lung and BAL fluid CD45+ (leukocytes) and CD45− (non-leukocytes) IL-6 producing cells in steady state (black bars) and 48 h after last HDM introduction (gray bars) in WT mice assessed by flow cytometry. (B) Mean fluorescence intensity (MFI) of non-hematopoietic (CD45−, blue histograms) and hematopoietic (CD45+, red histograms) IL-6 expressing cells in lungs and BAL fluid in steady state and after HDM introduction. (C) Number of IL-6 producing cells in BALF and lungs in steady state (black bar) and 48 h after last HDM introduction (gray bar) in WT mice. (D) Representative percentage ratio of IL-6 producing cells of different lung leukocyte populations in steady state (left) and 48 h after last HDM introduction (right) in WT mice assessed by flow cytometry. Datasets were first tested for Gaussian distribution with the D'Agostino & Pearson omnibus normality test. Parametric or non-parametric One-way ANOVA with multiple comparisons tests was applied where appropriated. Data represent means ± SEM, 5–11mice in each group.