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. 2018 Sep 19;27(24):4303–4314. doi: 10.1093/hmg/ddy331

Figure 3.

Figure 3

mHTT knockdown restores Neat1L expression. Eight-week-old BACHD mice were injected into the striatal with AAV vectors encoding artificial miRNAs targeting mHTT or with AAVs expressing a non-targeting control microRNA (miCtl) sequence. Striatal tissues were harvested 16 weeks later for RNA extraction. (A)mHTT mRNA levels in striatal tissue lysates as assessed by RT-qPCR (n = 3). Data are mean ± SEM and significance was analyzed by unpaired, two-tailed t-test, **P < 0.01. (B)Neat1L RNA levels in striatal tissue lysates from untreated WT mice or AAV.miHDS1 or AAV.miCtl-treated HD mice as assessed by RT-qPCR (n = 3). Data are mean ± SEM and significance analyzed by one-way ANOVA with Bonferroni post hoc analysis, *P < 0.05; ns = not significant. (C)Hotair RNA levels in striatal tissue lysates as assessed by RT-qPCR (n = 3). Data are mean ± SEM and significance analyzed by one-way ANOVA with Bonferroni post hoc analysis for panel B and C, ns = not significant.