Table 3.
Summary of the relationship between spina bifida (SB) frequency and Grhl3 abundance among embryos carrying different combinations of endogenous Grhl3 allele and Grhl3 transgene
| Genotype & NTDs | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Grhl3 locus | -/- | ct/- | ct/ct | +/- | +/+ | ct/- | ct/ct | +/+ | -/- | ct/- | ct/ct |
| Grhl3 transgene | 0/0 | 0/0 | 0/0 | 0/0 | 0/0 | Tg/0 | Tg/0 | Tg/0 | Tg/Tg | Tg/Tg | Tg/Tg |
| SB (%) | 100 | 50 | 10 | 0 | 0 | 0 | 0 | 15 | 38 | 70 | 70 |
| Increasing Grhl3 expression → | |||||||||||
| Grhl3 expression | 0 | ∼0.5 | ∼0.5 | 1 | ∼1.5 | ∼2 | 2.5-3 | ||||
Indicative expression levels (approximate) are based on collation of qRT-PCR data within differing experimental crosses. The frequency of spina bifida (SB) refers to approximate percentage of embryos with SB among each specific genotype.