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. 2018 Nov 2;37(23):e99552. doi: 10.15252/embj.201899552

Figure 4. PIF4 mediates de‐repressed expression of BR biosynthetic genes.

Figure 4

Col‐0 (black), PIF4‐OX (red), and pifq (blue) seedlings were grown for 6 days under short days, and samples were harvested at the indicated time points. Total RNA was used for qRT–PCR quantification of PIF + BES‐UP and the BR biosynthetic BES‐DOWN transcripts, using PP2A as endogenous control. Error bars represent SD of three technical replicates. Dark periods are shown in gray. Relative expression of the XTR7, PRE5, IAA19, and BR6ox2 genes in PIF4‐OX lines is referred to the right y axis of the graph. The experiment was repeated three times with similar results.