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. 2018 Nov 2;37(23):e99552. doi: 10.15252/embj.201899552

Figure 5. PIF4 activates expression of BR‐synthesis genes in response to elevated temperatures.

Figure 5

  1. Col‐0 and pifq seedlings were grown under short days at 22 and 28°C, and samples were collected at the indicated time points. Expression of BR biosynthetic genes was analyzed by qRT–PCR, using the constitutive PP2A gene as internal control. Error bars represent SD of three technical replicates. The experiment was repeated twice with similar results.
  2. Activation of the XTR7 PIF + BES‐UP target was studied as a positive control of the experiment. Error bars represent SD of three technical replicates. Dark periods are shown in gray. The experiment was performed three times with similar results.
  3. Increased levels of dephosphorylated BES1 at elevated temperatures depend on PIFs activation of BR synthesis. Col‐0 and pifq seedlings were grown for 6 days under short days and 22°C or 28°C, on half strength MS media (mock) or media supplemented with 0.5 μM BRZ. BES1 was analyzed by Western blot hybridization with an anti‐BES1 antibody. RPT5 was used as a loading control.

Source data are available online for this figure.