Figure 3.
Normal tongue morphology in Rgs21-null mice. Tongues of wild-type (n = 6; e.g., A) and Rgs21Δ5/Δ5 mice (n = 6; e.g., B) were stained with methylene blue to visualize the fungiform papillae on the anterior portions. There was no difference found in mean fungiform papillae number or morphology between Rgs21-null and wild-type animals (C; unpaired Student’s t test; P = 0.8254). Circumvallate papillae of wild-type (n = 4) and Rgs21Δ5/Δ5 mice (n = 4) were observed by bright-field microscopy (D, E; areas plotted in F). There was no difference found in circumvallate papillae area (F; unpaired Student’s t test; P = 0.8578).