Table 2.

Summary of Diagnostic Evaluation of Thyroid Tissue in Zebrafish Submitted for Histopathology During Iodine Deficiency (June 2016) and Recovery

Date	No. of fish examined	No. of tanks represented	Background	Age (days)	Grossly visible masses?	Thyroid carcinoma	Thyroid adenoma	Thyroid hyperplasia	No abnormal thyroid findings	Notes and additional findings
June 2016	12	8	WT, Mt, Tg	153–522	Yes	2	8	3	0	One fish hyperplasia in pharynx and adenoma at vent
August 2016	30	9	WT, Mt, Tg	251–909	Yes	0	3	23	4
October 2016	30	12	WT, Mt, Tg	227–970	Yes	0	1	18	11
December 2016	30	12	WT, Mt, Tg	311–879	Not reported	0	0	15	15
May 2017	19	3	Original WT	363–494	Not reported	0	0	0	19
May 2017	10	1	Original p53 Mt	550	Not reported	0	0	0	10	Two peripheral nerve sheath tumors, two sarcomas, one carcinoma
May 2017	20	3	New WT	85–168	Not reported	0	0	0	20
May 2017	10	1	New p53 Mt	74	Not reported	0	0	0	10

Original WT and p53 Mt refers to fish that were in the facility before the change to a higher iodine salt. New WT and p53 Mt refers to fish that were fertilized after salt change.

Mt, mutant; Tg, transgenic; WT, wild-type.