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. 2018 Nov 19;14(11):e1007437. doi: 10.1371/journal.ppat.1007437

Fig 2. Inhibition of SIRT2 enhances intracellular bacterial survival and CD8+T cell response.

Fig 2

A. Percentage survival of intracellular Salmonella in DCs in gentamicin protection assay in the presence and absence of SIRT2 inhibitor, AK7. (Mock- DMSO treated, AK7- 10 μM AK7 treated). (Data are presented as mean ± SEM of 4 independent experiments) B. 3[H] Thymidine incorporation to assess CD8+ T cells proliferation during Salmonella infection in response to SIRT2 inhibition. (Ova- Ovalbumin, DC- Dendritic cells, T cell- mixed lymphocyte, STM- Salmonella Typhimurium, AK7- 10 μM AK7, ConA- Concanavalin A) (Data are presented as mean ± SEM of 3 independent experiments) C. IL-2 levels during CD8+T cells proliferation assay during Salmonella infection in response to SIRT2 inhibition. (Ova- Ovalbumin, DC- Dendritic cells, T cell- mixed lymphocyte, STM- Salmonella Typhimurium) (Data are presented as mean ± SEM of 2 independent experiments) D. Percentage survival of intracellular Salmonella in DCs in gentamicin protection assay in wild type and SIRT-/- DCs. (Data are presented as mean ± SEM of 3 independent experiments) E. 3[H] Thymidine incorporation to assess CD8+ T cells proliferation during Salmonella infection in wild type and SIRT2-/- DCs. (Ova- Ovalbumin, DC- Dendritic cells, T cells- mixed lymphocyte, STM- Salmonella Typhimurium, ConA- Concanavalin A) (Data are presented as mean ± SEM of 3 independent experiments) F. IL-2 levels during CD8+ T cells proliferation assay in response to Salmonella infection in wild type and SIRT2-/- DCs. (Ova- Ovalbumin, DC- Dendritic cells, T cells- mixed lymphocyte, STM- Salmonella Typhimurium) (Data are presented as mean ± SEM of 2 independent experiments) (unpaired two-tailed Student’s t- test, p- value, *** < 0.0001, **<0.001, *<0.01).