Fig 4. SIRT2 is upstream of NOS2.
A. Representative immunoblot of NOS2 and SIRT2 in wild type and NOS2-/- DCs at the indicated time point. (UI- uninfected, STM-Salmonella Typhimurium infected) B. Densitometry analysis of SIRT2.1 in wild type and NOS2-/- DCs at the indicated time point. (UI- uninfected, STM-Salmonella Typhimurium infected). (Data are presented as mean ± SEM of 2 independent experiments) C. Densitometry analysis of SIRT2.2 in wild type and NOS2-/- DCs at the indicated time point. (UI- uninfected, STM-Salmonella Typhimurium infected). (Data are presented as mean ± SEM of 2 independent experiments) D. Representative immunoblot of NOS2 and SIRT2 in wild type and SIRT2-/- DCs at the indicated time point. (UI- uninfected, STM-Salmonella Typhimurium infected) E. Nitric oxide levels in conditioned media in response to Salmonella infection at 20 h post infection. (UI- uninfected DCs, STM- Salmonella Typhimurium infected DCs) (Data are presented as mean ± SEM of 3 independent experiments). (unpaired two-tailed Student’s t-test, p-value, *** < 0.0001, **<0.001, *<0.01).