Skip to main content
. 2018 Nov 19;14(11):e1007462. doi: 10.1371/journal.pgen.1007462

Fig 8. Ydj1/HDJ2 support RNR activity in yeast and mammalian cells.

Fig 8

(A) In yeast, Ssa1, Hsp82 and Ydj1 bind and stabilize the RNR complex allowing dNTP synthesis required cell cycle progression and DNA repair. Loss of Ydj1 in yeast results in lowered Rnr2 levels (increased Rnr2 degradation) and Rnr4 (decreased RNR4 transcription). (B) In mammalian cells, HSP70, HSP90 and HDJ2 bind and stabilize the RNR complex allowing dNTP synthesis required cell cycle progression and DNA repair. Loss of HDJ2 activity (through either CRISPR-mediated deletion or 116-9e) promotes lowering of R2B levels, sensitizing cells to RNR-inhibiting agents such as HU and triapine.