Fig. 1.

ZAG overexpression reduces body weight and alleviates obesity. A: Three phenotypes of mice, epididymal fat mass and hematoxylin and eosin (HE) staining (n = 3 per group) of epididymal fat tissue from the ND group, the HFD group, and the HFZ group. B: Changes in body weight over the ZAG recombinant plasmid treatment period. Body weight (C) and epididymal fat weight, perinephric fat, weight, and gastrocnemius muscle weight (E) of mice from the three groups (n = 8 per group). D: Food intake during ZAG recombinant plasmid treatment period. F: Concentrations of TG and NEFA in gastrocnemius muscle of mice from the three groups (n = 8 per group). The ND group was fed with ND, whereas the HFD and HFZ groups were fed with HFD. The results are expressed as mean ± SEM. Different letters indicate significant differences between groups (P < 0.05). Error bars indicate SEM.