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. 2018 Oct 14;59(12):2277–2286. doi: 10.1194/jlr.M082180

Fig. 4.

Fig. 4.

ZAG overexpression increases lipolysis and UCP3 expression via the β-AR/cAMP/PKA pathway in skeletal muscle. A: Western blot analysis of ZAG in plasma of mice from the three groups (n = 6 per group). B: The mRNA expression of β2-AR and β3-AR (n = 6 per group). Western blot analysis revealed accumulation of β2-AR and β3-AR (C); PKA, HSL, p-HSL, and ATGL (D); and C/EBPβ and FASN (F) in the gastrocnemius muscle of mice from the three groups (n = 6 per group). HSL concentrations (E) in the gastrocnemius muscle of mice from the three groups (n = 6 per group). The Western blot analysis (G) and mRNA expression (H) of PGC1α and UCP3 in the gastrocnemius muscle of mice from the three groups (n = 6 per group). The ND group was fed with ND, whereas the HFD and HFZ groups were fed with HFD. Values are expressed as mean ± SEM. Different letters indicate significant differences between groups, as determined (P < 0.05). Error bars indicate SEM.