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ZAG overexpression restores physiological parameters. After the ZAG recombinant plasmid treatment, individually housed mice were monitored for VO2 (A) and its area under curve (AUC) (B), VCO2 (C) and its AUC (D), RER (E), average value of RER (F), and energy expenditure (G) and its AUC (H) among the three groups (n = 5 per group). Values are expressed as mean ± SEM. Different letters indicate significant differences between groups, as determined (P < 0.05). Error bars indicate SEM.
