Fig. 1.

Effect of ROS inhibition on cytokine induced superoxide production in 3T3-L1 adipocytes and adipose explants. Differentiated 3T3-L1 adipocytes (A) and adipose explants (C) were treated with a cytokine mix (IL-1β, IFNγ, and TNFα) for 8 or 24 h. DPI (10 μM) was added the last hour of treatment. B: Undifferentiated fibroblast and differentiated adipocyte 3T3-L1 cells were tested for cytokine-induced superoxide production after 24 h of treatment with cytokine mix. Results are represented as mean ± SEM of three independent experiments. * P < 0.05; ** P < 0.01; *** P < 0.001 as compared with control (A, B) or cytokines (C); #P < 0.001 as compared with cytokines.