Fig. 2.

T317, GW3965, and 25HC inhibit infection of MLV-(VSV)-GFP pseudotyped viruses in HepG2 cells in an LXR-dependent manner. A, B: HepG2 cells were pretreated with synthetic LXR ligand (T317 or GW3965) or 25HC at the indicated concentrations for 24 h followed by infection with MLV-(VSV)-GFP pseudotyped viruses in the absence or presence of LXR ligand for 16 h. C, D: HepG2 cells were transfected with LXRα/β or CH25H siRNA (50 nM of each) for 24 h, and then infected with MLV-(VSV)-GFP pseudotyped viruses plus T317 or 25HC treatment as indicated. The viral infection was determined by the image assay (A, C) or the FACS assay (B, D). *P < 0.05 versus control in the corresponding groups (n = 6).