The ER stress-mediated signaling was altered in BAT of Th+/− mice in thermoneutrality conditions. A: Western blot analysis and quantification of CHOP protein. Ponceau Red was used as a loading control. B, C: Western blot analysis and quantification of pIRE or pJNK, respectively. Tubulin, total JNK, and vinculin were used as loading controls. All data are relative to Th+/+ mice densitometry at 29°C. Data are mean ± SEM. n = 4–10/group in A, and n = 3–10/group in B. Two-way ANOVA was used. *P < 0.05; **P < 0.01. a.u., arbitrary units.