Fig. 7.

The elongation of AA-d₈ and EPA-d₅ following ELOVL5 knockdown in Jurkat cells, proliferating primary T-cells, and HepG2 cells. Jurkat cells (A), proliferating T-cells (B), and HepG2 cells (C) were transfected with NS control siRNA and siRNA against ELOVL5. Cells were incubated for 48 h in regular culture media before a 24 h supplementation with 5 µM AA-d₈ or EPA-d₅. Cellular lipids were extracted and hydrolyzed, and pentafluorobenzyl esters of FAs were prepared and measured by negative ion chemical ionization GC/MS. The values represent the percentage of the cellular AA-d₈ and EPA-d₅ that was elongated to 22:4-d₈ and 22:5-d₅, respectively. The results are means ± SEMs of three independent experiments. *Different from cells transfected with the NS siRNA (P < 0.05) as determined by paired two-sided Student’s t-test.