Fig. 8.

Effect of OEA on cellular and media triacylglycerol and phospholipid levels and cellular MTP activity and mRNA levels in PPARα-deficient primary enterocytes. Primary enterocytes were isolated from the proximal intestines of WT and Pparα knockout mice and incubated in triplicate without (−OEA) or with 200 μM OEA (+OEA) in DMSO in the presence of 2.5 μCi/ml of [³H]glycerol. Comparisons were made between the +OEA- and −OEA-treated groups. A: For uptake studies, cells were collected at indicated times and radioactivity was measured. B: To determine secretion rates, cells were pulse labeled with [³H]glycerol for 2 h, washed, and incubated in label-free media. At indicated times, radioactivities in media were quantified. C–F: Cells were treated with or without 200 μM OEA in DMSO in the presence of 2.5 μCi/ml of [³H]glycerol for 2 h, washed, and incubated in label-free media for 2 h. Lipids were extracted from cells and media, separated on TLC, and triacylglycerol (C, E) and phospholipid (D, F) bands were quantified. G–L: Enterocytes were incubated in triplicate with or without OEA for 4 h. MTP activity (G) and different mRNA levels (H–L) were measured in cells. The data are plotted as mean ± SD, n = 3. ***P < 0.001 as compared with the no OEA group.