Fig. 1.

Protein interaction of ABHD5 with PNPLA1 indicates a direct role of ABHD5 in AcylCer biosynthesis. A: Molecular structure of AcylCer. B: Proposed pathway of AcylCer biosynthesis. Transacylation of linoleic acid from TAG molecules onto ULC ω-hydroxy ceramides requires the presence of PNPLA1 and ABHD5. C: His-tagged ABHD5 coimmunoprecipitates with FLAG-tagged PNPLA1. Recombinant proteins were detected in cell homogenates (input) and anti-FLAG IPs by immunoblot analysis using adequate antibodies. D: Coomassie blue staining indicates equal protein loading. Cer, ceramide; DAG, diacylglycerol; FA, fatty acid; IP, immunoprecipitate; LC, long chain; VL, very long chain; ω-OH, omega-hydroxy.