Fig. 1.

Design of MDM2/X-Obstructing Protein (MOP) constructs. (A) Sequence of optimized ¹⁰FN3 (OPT¹⁰FN3). Position of the D7K mutation is indicated by *. Loop sequences that were replaced in our work are underlined. ▼ Represents the position within loop sequences where an insertion was made. (B) Ribbon representation of ¹⁰FN3. ¹⁰FN3 (PDB ID: 1FNF) shown in blue with exposed loops BC, DE, FG, AB, CD and EF colored in magenta and labeled accordingly. (C) Panel of tested peptide sequences comprised of M2 sequence in green with varying lengths of flanking GS-rich spacers. The optimal spacer combination is colored in pink.