Fig. 3.

Cellular inhibition of MDM2/X in T22 cell-based assay. (A) A comparison of the effect of lead engineered FG loop ¹⁰FN3 domain SGGG-M2-GGGS (MOP3) with SG-M2-GG on T22 reporter activity in transiently transfected T22 cells. SG-M2C-GG and SGGG-M2C-GGGS (MOP3C) are engineered negative control ¹⁰FN3 domains with mutation of key interacting residues. Reporter activity was determined 24 h after transfection. The results shown depict fold p53 reporter activation relative to EGFP alone. Data represent mean ± SD (n = 2). (B) Comparison of engineered AB/CE/EF loop ¹⁰FN3 domain to lead engineered FG loop ¹⁰FN3 domain (MOP3) in T22 reporter assay. Data represent mean ± SD (n = 2).