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MOP3 and MOP3+ bind endogenous MDM2 and MDMX. p53-positive (HCT116 p53+/+) or p53-null (HCT116 p53−/−) cells were transfected with respective plasmids expressing EGFP-fusion and control constructs. Lysates were immunoprecipitated with anti-EGFP agarose beads and probed for (A) MDM2 or (B) MDMX.
