Fig. 8.
Live cell spinning disk confocal fluorescence microscopy of stable T22 expressing EGFP-MOP3 in the presence of other MDM2 inhibitors. After 5 h of doxycycline induction, sMTIDE-02 (25 μM), sMTIDE-02SCRAM (25 μM) or Nutlin (10 μM) were added. Four z-stacks were immediately taken at time = 0 and every 2.5 min thereafter. Selected time points for the respective treatments were shown as indicated at the upper left corner of each panel in min:s. Each panel is a projection of sum slices of the z-stacks (ImageJ). White arrowheads were added to aid in visualization of selected cells through the time course.