Table I.
Oligos | Nucleotide sequeces in 5′–3′ direction |
---|---|
INF-10FN3-ndeF | AAGGAGATATACATATGGTTTCTGATGTTCCGCGTAAGC |
INF-10FN3-HA-bamR | GCTCGAATTCGGATCCTCAAGCGTAATCTGGAACATCGTATGGGTACTCGAGCGCGGTACGGTAGTTAATCG |
FNR1 | GTACTCGGCGAAGCTAGTAACAGCGTATACAGTGATGGT |
FNF3 | TGGGCCCTGCTGAGCGGAGGAGGATCTCCAATCTCGATTAACTACCGTAC |
FNC-R1 | GTACTCGGCAGCGCTAGTAACAGCGTATACAGTGATGGT |
FNC-F3 | GCTGCCCTGGCTAGCGGAGGAGGATCTCCAATCTCGATTAACTACCGTAC |
SGGG-Fstrand-10FN3-R | TCCTCCTCCAGAAGTAACAGCGTATACAGTGATGGT |
PM1-F | AGCTTCGCCGAGTACTGGGCCCTGCTGAGC |
PM1ala-F | AGCGCTGCCGAGTACGCTGCCCTGGCTAGC |
PM2-CD-for2 | ATATTGGGCGCTGCTGAGCGGAGGAGGATCTGTTCAGGAGTTCACTGTACCTGGTTCCA |
PM2-CD-rev | TCCGCAAAGCTTCCTCCTCCAGAGGTTTCACCGTACGTGATACGGTAATAAC |
petF2 | CATCGGTGATGTCGGCGAT |
petR | CGGATATAGTTCCTCCTTTCAGCA |
INF-OPT10FN3-XhoI-pEGFP-C1-F | GGACTCAGATCTCGAGCTATGGTGAGTGACGTGCCTCGGAAAC |
INF-OPT10FN3-BamH1-pEGFP-C1-R | TAGATCCGGTGGATCCCTACTCCAGGGCTGTGCGGTAGTTTATTG |
OPT10FN3-FN3-F | GGAGGAGGATCTCCTATTTCAATAAACTACCGCACA |
OPT10FN3-SGGG-PMI-Rcor | GCTCAGCAGGGCCCAGTACTCGGCGAAGCTTCCTCCTCCAGATGTCACGGCGTAGACAGTGATGGT |
OPT10FN3-SGGG-PMIala-Rcor | GCTGCTAGCCAGGGCAGCGTACTCGGCAGCGCTTCCTCCTCCAGATGTCACGGCGTAGACAGTGATGGT |
10FN3-HIS-BamR | GCTCGAATTCGGATCCTCAGTGATGGTGATGGTGATGTCCTCCTCCAGACTCGAGCGCGGTACGGTAGT TAATCGAGATTGG |
INF-FIBpTET-EcoR1F | CCCTCGTAAAGAATTCATGGTGAGCAAGGGCGAGGAGCTGTTC |
INF-FIBpTET-BamH1R | GCAGAGATCTGGATCCCTACTCCAGGGCTGTGCGGTAGTTTATTG |
INF-pTET-EcoR1F | GAATTCTTTACGAGGGTAGG |
INF-pTET-BamH1R | GGATCCAGATCTCTGCAGC |
infus-Mdm2-F | AAGGAGATATACATATGTGCAATACCAACATG |
infus-Mdm2FLAG-R | GCTCGAATTCGGATCCTTATTTATCATCATCATCTTTATAATCGGGGAAATAAGTTAGCACAATCATT |
INF-pTet-attL/R4X-F | GCAGGAAAGAACATGTGAAGCCTGCTTTATTTCATTAAGTTGGCATTATAAAAAAGCATTGCTTATCAAT TTGTTGCAACGAACAGGTCACTATCAGTCAAAATAAAATCATTATTTGATTTCAATTTTGTCCCACTCCCT CCCGGACATGTGAGCAAAAGG |
INF-pTet-attL/R4X-R | CCTTTTGCTCACATGTCCGGGAGGGAGTGGGACAAAATTGAAATCAAATAATGATTTTATTTTGACTGAT AGTGACCTGTTCGTTGCAACAAATTGATAAGCAATGCTTTTTTATAATGCCAACTTAATGAAATAAAGCA GGCTTCACATGTTCTTTCCTGC |