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. 2018 Aug 30;31(7-8):301–312. doi: 10.1093/protein/gzy018

Table I.

Oligonucleotide sequences used for construction of vectors

Oligos Nucleotide sequeces in 5′–3′ direction
INF-10FN3-ndeF AAGGAGATATACATATGGTTTCTGATGTTCCGCGTAAGC
INF-10FN3-HA-bamR GCTCGAATTCGGATCCTCAAGCGTAATCTGGAACATCGTATGGGTACTCGAGCGCGGTACGGTAGTTAATCG
FNR1 GTACTCGGCGAAGCTAGTAACAGCGTATACAGTGATGGT
FNF3 TGGGCCCTGCTGAGCGGAGGAGGATCTCCAATCTCGATTAACTACCGTAC
FNC-R1 GTACTCGGCAGCGCTAGTAACAGCGTATACAGTGATGGT
FNC-F3 GCTGCCCTGGCTAGCGGAGGAGGATCTCCAATCTCGATTAACTACCGTAC
SGGG-Fstrand-10FN3-R TCCTCCTCCAGAAGTAACAGCGTATACAGTGATGGT
PM1-F AGCTTCGCCGAGTACTGGGCCCTGCTGAGC
PM1ala-F AGCGCTGCCGAGTACGCTGCCCTGGCTAGC
PM2-CD-for2 ATATTGGGCGCTGCTGAGCGGAGGAGGATCTGTTCAGGAGTTCACTGTACCTGGTTCCA
PM2-CD-rev TCCGCAAAGCTTCCTCCTCCAGAGGTTTCACCGTACGTGATACGGTAATAAC
petF2 CATCGGTGATGTCGGCGAT
petR CGGATATAGTTCCTCCTTTCAGCA
INF-OPT10FN3-XhoI-pEGFP-C1-F GGACTCAGATCTCGAGCTATGGTGAGTGACGTGCCTCGGAAAC
INF-OPT10FN3-BamH1-pEGFP-C1-R TAGATCCGGTGGATCCCTACTCCAGGGCTGTGCGGTAGTTTATTG
OPT10FN3-FN3-F GGAGGAGGATCTCCTATTTCAATAAACTACCGCACA
OPT10FN3-SGGG-PMI-Rcor GCTCAGCAGGGCCCAGTACTCGGCGAAGCTTCCTCCTCCAGATGTCACGGCGTAGACAGTGATGGT
OPT10FN3-SGGG-PMIala-Rcor GCTGCTAGCCAGGGCAGCGTACTCGGCAGCGCTTCCTCCTCCAGATGTCACGGCGTAGACAGTGATGGT
10FN3-HIS-BamR GCTCGAATTCGGATCCTCAGTGATGGTGATGGTGATGTCCTCCTCCAGACTCGAGCGCGGTACGGTAGT TAATCGAGATTGG
INF-FIBpTET-EcoR1F CCCTCGTAAAGAATTCATGGTGAGCAAGGGCGAGGAGCTGTTC
INF-FIBpTET-BamH1R GCAGAGATCTGGATCCCTACTCCAGGGCTGTGCGGTAGTTTATTG
INF-pTET-EcoR1F GAATTCTTTACGAGGGTAGG
INF-pTET-BamH1R GGATCCAGATCTCTGCAGC
infus-Mdm2-F AAGGAGATATACATATGTGCAATACCAACATG
infus-Mdm2FLAG-R GCTCGAATTCGGATCCTTATTTATCATCATCATCTTTATAATCGGGGAAATAAGTTAGCACAATCATT
INF-pTet-attL/R4X-F GCAGGAAAGAACATGTGAAGCCTGCTTTATTTCATTAAGTTGGCATTATAAAAAAGCATTGCTTATCAAT TTGTTGCAACGAACAGGTCACTATCAGTCAAAATAAAATCATTATTTGATTTCAATTTTGTCCCACTCCCT CCCGGACATGTGAGCAAAAGG
INF-pTet-attL/R4X-R CCTTTTGCTCACATGTCCGGGAGGGAGTGGGACAAAATTGAAATCAAATAATGATTTTATTTTGACTGAT AGTGACCTGTTCGTTGCAACAAATTGATAAGCAATGCTTTTTTATAATGCCAACTTAATGAAATAAAGCA
GGCTTCACATGTTCTTTCCTGC