Table II.

Loop sequences in engineered single or bivalent ¹⁰FN3 domain inhibitors

Constructsa	AB loop	CD loop	EF loop	FG loop
WTb	TPTS	GGNSP	GLKPG	GRGDSPASSK
FG (MOP3)				SGGGSFAEYWALLSGGGSc
FGC (MOP3C)				SGGGSAAEYAALASGGGSd
AB	SGGGSFAEYWALLSGGGS
AB2	TPSFAEYWALLSTS
AB:FG	SGGGSFAEYWALLSGGGS			SGGGSFAEYWALLSGGGS
AB2:FG	TPSFAEYWALLSTS			SGGGSFAEYWALLSGGGS
CD		SGGGSFAEYWALLSGGGS
CDC		SGGGSAAEYAALASGGGS
CD:FG (MOP3+)		SGGGSFAEYWALLSGGGS		SGGGSFAEYWALLSGGGS
CDC:FG		SGGGSAAEYAALASGGGS		SGGGSFAEYWALLSGGGS
EF			SGGGSFAEYWALLSGGGS
EF2			GLSFAEYWALLSKPG
EF: FG			SGGGSFAEYWALLSGGGS	SGGGSFAEYWALLSGGGS
EF2:FG			GLSFAEYWALLSKPG	SGGGSFAEYWALLSGGGS

^aName of ¹⁰FN3 domain inhibitors based on loop where a replacement (AB, CD, EF or FG) or insertion was made (AB2, EF2).

^bWild-type sequences of AB, CD, EF and FG loop region.

^cSGGGSFAEYWALLSGGGS = SGGG-M2-GGGS.

^dSGGGSAAEYAALASGGGS = SGGG-M2C-GGGS.