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. 2018 Jul 14;12(3-4):197–203. doi: 10.1080/19336896.2018.1471922

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Figure 4. — Electropherogram of c.268_269insC polymorphism in chicken PRNP gene. Four colors indicate individual bases of DNA sequence using ABI 3730 automatic sequencer (blue: cytosine, red: thymine, black: guanine, green: adenine). Sanger sequencing was performed by reverse gene-specific primer, as described in the materials and method section. Arrows indicate c.268_269insC polymorphism found in this study. Upper portion: heterozygote of c.268_269insC polymorphism. Lower portion: wild-type of the chicken PRNP gene sequence. WT: wild-type of the chicken PRNP gene sequence. Insertion: c.268_269insC polymorphisms of the chicken PRNP gene sequence.