Table 2.

Measurement of the effect of substitutions from horse-specific amino acids to interspecific conserved amino acids on horse prion protein.

Residues	Horse specific	Interspecific conserved	Methods	Score	Prediction
169	S	D	PolyPhen-2	0.000	Benign
			PROVEAN	0.515	Neutral
			PANTHER	2	Probably benign
224	Y	S	PolyPhen-2	0.005	Benign
			PROVEAN	0.977	Neutral
			PANTHER	2	Probably benign
227	F	Y	PolyPhen-2	0.000	Benign
			PROVEAN	0.908	Neutral
			PANTHER	2	Probably benign
228	Q	Y	PolyPhen-2	0.000	Benign
			PROVEAN	0.861	Neutral
			PANTHER	2	Probably benign
243	V	I	PolyPhen-2	NA	Unknown
			PROVEAN	0.068	Neutral
			PANTHER	2	Probably benign

* NA, Not available