Video 13. Orthoview of a second mitosis with two horizontal-vertical re-orientations.

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DOI: 10.7554/eLife.36248.033

The top panel shows condensed chromatin of the dividing cell (ubi-his2ab::mRFP). The red line indicates the spindle axis. The cyan line indicates the basal plane, as revealed by the basement membrane stain Concanavalin A-Alexa 647 (not shown). The bottom panel reproduces the graph from Figure 4E, with the time-resolved orientations of this particular cell in red. The first re-orientation occurs during metaphase (from 16° at 5 min to 0° at 15 min). The second re-orientation occurs between metaphase (1° at 20 min) and anaphase (18° at 25 min). Scale bar, 5 µm.