Video 17. A high-contact sibling pair (Pair A, Figure 6A,C) does not activate GBE-Su(H)-GFP:nls.

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DOI: 10.7554/eLife.36248.037

Following their birth at t=0.0 hr, the two siblings probably remain in contact (inter-nuclear distance <6.0 µm; c.f. Figure 6—figure supplement 1) for at least 6.0 hr. The mother stem cell is indicated by the blue dotted circle at t=−1.2 hr; the two siblings are indicated by the two blue dotted circles at t=0.0 and t=6.0 hr. No GFP expression is apparent in either sibling. Left video: green, GBE-Su(H)-GFP:nls; magenta, stem cell and enteroblast nuclei (esg >his2b::CFP); gray, all nuclei (ubi-his2av::mRFP). Right video: inverted gray, GBE-Su(H)-GFP:nls. Each time point is the partial projection of a confocal stack. Scale bar, 5 µm.