Video 2. Movie showing the association of the trachea (cyan) with the midgut tube (red).

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DOI: 10.7554/eLife.36248.008

Smaller tracheal branches encircle the tube and move in concert with peristaltic contractions. A large tracheal branch (upper right) is continuous with smaller branches. The large branch does not move during peristalsis because it is not physically associated with the midgut; instead, it connects the midgut-associated branches to a spiracle (not visible in the movie frame). Left video: cyan pseudocolor, trachea (breathlessGal4, UAScyt-GFP); red pseudocolor, microtubules (SiR-tubulin). Right video: inverted gray, breathlessGal4, UAScyt-GFP. Each time point is the projection of a confocal z-stack. Scale bar, 20 µm.