Video 8. Twelve-hour movie of a single-enterocyte extrusion.
The epithelium is oriented with its basal surface toward the microscope objective and its apical surface further away. The basal region of the extruding enterocyte (orange pseudocolor at t=0, 127.5, 285, 442.5 min) is outlined by a ‘ring’ of E-cadherin::YFP. The ring closes down to a point from t=255–442.5 min. The intensity of the ring fluctuates during the first half of closure and becomes consistently bright during the second half. As the ring closes, neighboring cells draw into a rosette. Meanwhile, the nucleus of the extruding cell (yellow pseudocolor) starts to drop apically at t=150 min, hits its deepest luminal position at t=262.5 min, and recoils from t=262.5–307.5 min. Cyan, all nuclei (ubi-his2av::mRFP); inverted gray, E-cadherin (ubi-DE-cadherin::YFP). Each time point is the projection of a confocal stack. Scale bar, 10 µm.