Video 9. Orthoview of extrusion shown in Video 8.

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DOI: 10.7554/eLife.36248.029

The nucleus of the extruding enterocyte (magenta) ejects out of the epithelium (t=150–165 min) and penetrates into the lumen (t=165–265 min). It subsequently recoils and eventually comes to rest on the apical epithelium (t=263–443 min). The multicolored line shows the path of nuclear travel over time (violet-yellow color scale; see Figure 3D for legend). Cyan, all nuclei (ubi-his2av::mRFP); gray, E-cadherin (ubi-DE-cadherin::YFP). Scale bar, 10 µm.