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NOx responses in RAW 264.7 macrophages treated with LPS. Cells were cultured in DMEM media with or without LPS for ten hours, after which NOx was measured in the cell media (A, B) and in the cell lysates (C, D) with triiodide and PBS/FeCN. Note the 30-fold increase in cell media NOx as measured by triiodide demonstrating successful iNOS stimulation. Data are presented as mean ± SEM, n = 3, *= p < 0.05, **= p < 0.01, *** = p < 0.001.
