Table 1.

α-SMA ELISA Technical Validation Data

Technical Validation Test	Result
IC50	3.43 ng/ml
Detection range	0.44-25.5 ng/ml
Intra-assay variation⁎	8.0%
Inter-assay variation⁎	13.0%
Dilution recovery of human serum⁎	99%
Dilution recovery of human urine⁎	82%
Dilution recovery of rat serum⁎	84%
Dilution recovery of rat urine⁎	90%
Analyte recovery 24 h, 4°C/20°C⁎	102%/97%
Hemoglobin recovery⁎	86%
Lipemia recovery⁎	99%
Analyte recovery, 4 freeze/thaw cycles⁎	97%
Salt recovery, pH 7.0/pH 8.0†	99%

^⁎Percentages are reported as mean.

^†Average recovery after salt interference.